Key Features

• Reliable ResultsThe targeted PCR-based assay deploys small amplicons sourced from the latest mitochondrial (mtDNA) databases for enhanced variant detection. A tiled primer approach overlaps the amplicons to prevent the sequence gaps that can lead to data loss, particularly in degraded samples. Complementing this tiled primer design, an enhanced buffer system delivers exceptional resistance to calcium, humic acid, and other relevant PCR inhibitors.
• Flexible Workflow OptionsThe protocol includes two normalization methods to tailor library prep for a broad range of input material, from high-quality genomic DNA (gDNA) through low-level and complex samples. A low input requirement expands possibilities for the most challenging samples. For efficient use of the reagent kit and accurate read assignment, index adapters provided in the library prep kit allow pooling and sequencing of up to 16 samples in one run.
• Complex Bioinformatics, SimplifiedThe ForenSeq mtDNA Whole Genome Kit pairs seamlessly with the ForenSeq mtDNA Analysis Module in UAS for ease-of-use and rapid review of mtDNA data. The software analyzes sequencing output for the whole mitochondrial genome and provides results less than one hour after run completion. Laboratories can review summarized or detailed results and compare up to nine samples on a clear and interactive user interface. Reports are compatible with multiple DNA databases.

Specifications