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SpectraMax iD3 Multi-Mode Microplate Reader

Identifiably Different

The SpectraMax® iD3 Multi-Mode Microplate Reader is the cornerstone of a complete laboratory solution to help you expand the boundaries of your research capabilities. With optimized reagents and the industry-leading data acquisition and analysis tool SoftMax® Pro 7 Software, the SpectraMax iD3 allows you to customize your workflow to perfectly match your needs.

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Overview

 

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LARGE TOUCHSCREEN
The SpectraMax iD3 Multi-Mode Microplate Reader features a large, high-resolution touchscreen with an embedded software package allowing you to set up custom protocols, take advantage of preconfigured protocols, and run your experiment without the need for a dedicated computer workstation.

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Personalized workflows

A built-in near-field communication (NFC) reader enables you to pull up your custom protocols with a single tap, saving you precious time. Pair NFC Tags with specific user profiles to gain access to personalized protocols and experiment results eliminating the need to scroll through dozens of files.

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Increased sensitivity

The superior optical system includes a xenon flash lamp and features an ultra-cooled photomultiplier tube (PMT) to -5°C that reduces background noise for excellent sensitivity and a wide dynamic range.

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Flexible temperature control

Simple-to-use temperature control allows you to adjust your experiment's conditions from ambient up to 65°C, expanding your laboratory's capabilities to include temperature sensitive assays

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Data you need anywhere you want it

View your data quickly using the large touchscreen, export to a USB drive, or analyze your data using the industry-leading data acquisition and analysis tool, SoftMax Pro 7 Software. QuickSync technology allows you to walk away from the SpectraMax iD3 to focus on additional research. Data is automatically delivered to your workstation, eliminating the need to physically retrieve your data from the instrument.

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A complete solution to answer all your research needs

Featuring orbital shaking, a four-monochromator optical pathway with high efficiency gratings, well scanning up to a 20x20 read matrix, spectral scanning and detection of plate formats from 6- to 384-wells, the SpectraMax iD3 reader is the complete solution to all your research needs.

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CAPTURE FLASH ASSAYS WITH EASE
Expand your lab’s capabilities to include flash applications such as dual luciferase and ATP assays with the SpectraMax iD3 injector system with SmartInject™ Technology ensuring equal mixing across the plate for high-precision experiments. Engineered for high performance, the dual injector system can be used with any read mode and includes safeguard features such as overflow protection to save precious reagents and maximize performance for those critical experiments in your lab

SMP button Compliance

SoftMax Pro GxP Compliance Software

SoftMax® Pro GxP Compliance Software extends Molecular Devices leading data acquisition and analysis solution into regulated laboratories working under GMP, GLP, 21 CFR Part 11, and other similar guidelines for secure electronic records.

Compatible Product

 

 

COMPATIBLE SOFTWARE:

CAPTURE FLASH ASSAYS WITH EASE
Expand your lab’s capabilities to include flash applications such as dual luciferase and ATP assays with the SpectraMax iD3 injector system with SmartInject™ Technology ensuring equal mixing across the plate for high-precision experiments. Engineered for high performance, the dual injector system can be used with any read mode and includes safeguard features such as oveSOFTMAX PRO 7 SOFTWARE
SoftMax® Pro Software is the most published microplate reader control and microplate data analysis software package available to scientists. The inclusive package of ready-to-run protocols, analysis algorithms, and 21 different curve fit options provides the full solution from data acquisition to analysis to publishing. The FDA 21 CFR Part 11 compliance tools available in SoftMax Pro GxP Software for regulated laboratories deliver a complete solution to minimize validation costs, provide end-to-end chain of custody, and eliminate the need to validate additional analysis software.ion to save precious reagents and maximize performance for those critical experiments in your lab

Specifications

General specifications
Dimensions (in.) 15.79 (H) x 20.94 (W) x 23.54 (D)
Dimensions (cm) 40.1 (H) x 53.2 (W) x 59.8 (D)
Weight 88.1 lbs. (40 kg)
Power requirements 100–240 VAC, 2 A, 50/60 Hz
Robotic compatible Yes
General performance
Plate formats 6 to 384 wells 
Light source Xenon flash lamp
Reading capabilities Microplates, cuvettes (via adapter)
Detectors Photo Multiplier Tube and Photodiode
Shaking Orbital, Infinity
Temp. control 5°C above ambient to 65°CŒ
Temp. uniformity ± 1°C
Temp. accuracy ±1°C at 37°C set point
Spectral scanning Abs, FI, Lum
Endpoint reading Abs, FI, Lum
Kinetic reading Abs, FI, Lum
Well scanning Over 20 by 20 in all modes
Wavelength selection 1.0 nm increments
Standard read times (minutes:seconds)
96 wells 384 wells
Absorbance 0:25 1:25
Fluorescence intensity 0:17 0:53
Luminescence 0:26 1:01
Absorbance photometric performance
Wavelength range 230–1000 nm
Wavelength bandwidth 4.0 nm
Wavelength accuracy ± 2.0 nm
Wavelength repeatability ± 1.0 nm
Photometric range 0–4.0 OD
Photometric resolution 0.001 OD
Photometric accuracy < ±0.010 OD ±1.0%, 0–3 OD
Photometric precision < ±0.003 OD ±1.0%, 0–3 OD
Stray light < 0.05% @ 230 nm
Fluorescence intensity performance
Wavelength range 250–850 nm
Wavelength selection 1.0 nm increments
Dynamic range > 6 logs
Optimized Top sensitivity (fluorescein)
96 wells 1 pM
384 wells 1 pM
Optimized Bottom sensitivity (fluorescein)
96 wells 2 pM
384 wells 2.5 pM
Luminescence performance
Wavelength range 300–850 nm
Wavelength selection Choice of simultaneous detection of all wavelengths or selection in 1.0 nm increments
Dynamic range > 7 decades
Cross-talk < 0.1% in white 96- and < 0.2% in 384-well microplates
Optimized Sensitivity (ATP-Glow)
96 wells 2 pM
384 wells 4 pM
ATP Concentration Graph
An ATP standard curve spanning five decades was run using the ATPlite 1step Luminescence Assay System (PerkinElmer) on the SpectraMax iD3 reader. Standard concentrations ranged from 1x10-11 M to 1x10-6 M. A wide linear dynamic range ensures accurate assay results across a broad span of sample types. Standards were plotted using a log-log curve fit in SoftMax Pro Software.
The SpectraMax iD3 reader is fully compatible with the SpectraDrop Micro-Volume Microplate, enabling quantitation of precious lowvolume samples. 4-uL DNA standards from 2 ng/μL to 1000 ng/ μL were read in absorbance detection mode with a preconfigured protocol in SoftMax Pro Software. Performance matching the 2 ng/μL sensitivity specification is demonstrated here.

 

 

Resources

Download Application Note

One of the most popular methods for assaying cell health is fluorescence detection with a microplate reader. In this application note, we report the use of the EarlyTox™ Cell Viability suite of assay kits on the SpectraMax® iD3 Multi-Mode Microplate Reader.
Click here to download the application note >>

 

 

 

Download SpectraMax iD3 Data Sheet

View SpectraMax iD3 Tutorial Videos

Getting to know your new SpectraMax iD3 Multi-Mode Microplate Reader

View a brief overview of the features and benefits of your new SpectraMax iD3 Multi-Mode Microplate Reader.
Click here to view >>

 

 

 

Personalizing your new SpectraMax iD3 Multi-Mode Microplate Reader

Learn how to personalize your SpectraMax iD3 Multi-Mode Microplate Reader.  Create user profiles and pair them to NFC tags to easily pull up saved protocols, and experiment results.
Click here to view >>

 

 

 

 

Starting a plate read on your new SpectraMax iD3 Multi-Mode Microplate Reader

Learn how to quickly and easily start a plate read using the high-resolution touchscreen interface.
Click here to view >>

 

 

 

Viewing results on your new SpectraMax iD3 Multi-Mode Microplate Reader

Tutorial on how to quickly and easily view your results on the SpectraMax iD3 Multi-Mode Microplate Reader.
Click here to view >>

 

 

 
 
 

Our Partners

Address

Bio-Active Co., Ltd. 
188/1 Bio-Active Bldg., Soi Sirung,
Chua Phloeng Rd., Chongnonsi,
Yannawa Bangkok 10120 
Thailand

 

ที่อยู่

บริษัท ไบโอแอคทีฟ จำกัด
188/1 อาคารไบโอแอคทีฟ ซอยศรีรุ้ง
ถนนเชื้อเพลิง แขวงช่องนนทรี
เขตยานนาวา กรุงเทพฯ 10120

Phone

  • Hot line: 66-2350-3090
    FAX : 66-2350-3080

 

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