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ImageXpress Micro 4 High-Content Imaging System

ImageXpress Micro 4 High-Content Imaging System


The NEW ImageXpress® Micro 4 High-Content Imaging System offers an agile solution enabling you to expedite your research.


ImageXpress Micro 4-298 1

The ImageXpress® Micro 4 High-Content Imaging System represents the culmination of four generations of imaging expertise. The latest agile design allows you to boost your research with this faster-than-ever system, while providing the option to upgrade to confocal in the future to align with your research needs.

Capture images of whole organism and cellular or intracellular events with the ImageXpress Micro 4 system configured to suit your specific biological needs.  We offer unlimited configurability with user exchangeable filter cubes, the widest range of objective lenses and environmental control, transmitted light, confocal imaging, and fluidics options.

Built on over 30 years of cell-based imaging experience, the ImageXpress Micro 4 High-Content Imaging system is engineered for performance to reliably accelerate the pace of your research


4th Generation Platform with unlimited configurability, developed to address your research needs.


4th Generation Platform with unlimited configurability, developed to address your research needs.


The automated 5-position filter cube changer facilitates multiplex assays with up to five filter sets and four objectives for a single experiment.


Minimize tiling effect and reduce your acquisition time with the ability to image a whole 384 well at once.


Available options include Environmental Control (temperature, humidity and CO2 control for multi-day live-cell experiments), Transmitted Light True Phase Contrast, Confocal Imaging (field upgradable), and Fluidics (on board disposable pipetting).


Investigate every aspect of a cellular pathway

Combined with MetaXpress High-Content Image Acquisition and Analysis Software, the ImageXpress Micro 4 system is an end-to-end solution that enables you to interpret your images, understand your data, and explore new ideas.

Automatically acquire images 1-100x objective magnification in slides and multi-well plates up to 1536-well format. Acquire >245,000 wells per day at 4x magnification with the system’s unique multi-well crop feature. A 4x objective captures 11 mm2 of a 384-well plate to be imaged in one field-of-view while increasing magnifications allows the resolution of fine detail for analysis of subcellular events.























  • Determine the mechanisms of action for the research of cancer, neurological disorders, viral infection, and other diseases with compound or RNAi screening 
  • Quantitate multiplexed cellular responses, such as apoptosis, cell cycle, micronuclei, neurite outgrowth, angiogenesis, intracellular translocation, endocytosis, and cell signaling
  • Detect one to four fluorescent markers along with the ability to resolve their colocalization

    Further InformationVisit the MetaXpress Software Application Modules to learn more about analysis for cell health, cell cycle, micronuclei, neurite outgrowth, angiogenesis, translocation, multi-wavelength cell scoring and granularity.


Interrogate large-scale RNAi librariesMeasure critical events including apoptosis, cell cycle, micronuclei, neurite outgrowth, angiogenesis, intracellular translocation, endocytosis, cell signaling, and multi-wavelength cell scoring.Automatically identify, segment, and analyze biological phenotypes using validated software modules

imagexpressmicro.rnai h2ax 450 0 1


Images of control cells showing DNA damage detected by H2AX phosphorylation. The positive control (bottom) clearly shows a higher percentage of cells with DNA damage (green) compared to total cells (red) in the well.

Further Information

  • Measure stem cell growth and differentiation in layers or colonies with the system's widefield focusing capabilities, brightfield imaging option, and environmental control chamber for extended period of time
  • Accurately track cell processes, colony size or number across multiple fields of view with unique stitching algorithm and precise plate stage movements
  • Gain insight into the complex molecular and genetic signaling events that underlie cell proliferation and differentiation
  • Accurately measure nuronal process across multiple fields of view using unique stitching algorithm with precise stage movements
  • Analyze 3D samples by capturing an image stack in the Z-plane within each field of view and collapse it into a best-focus image or view the sample in 3D

imagexpress-micro-neuronalstemcells 1Differentiated neurons imaged in a 96-wellplate at 10x magnification with three different
fluorescent markers

Further Information

{slide=Live Cells}

  • Monitor cell growth, death, differentiation, and migration; viral or bacterial invasion, cancer metastasis, chemotaxis, drug toxicity, or translocation
  • Observe rapid kinetic cellular responses over a span of several days using fluorescent or unlabeled cells kept in an environmental control chamber, together with a brightfield microscope, and on-board fluid addition capabilities


Watch a 30-hour time-course movie of increasing apoptotic and dead HeLa cells after treatment with 100 µm Etoposide. Blue = Hoechst for all nuclei; Red = Propidium Iodide for dead cells; Green = Nucview488, a fluorescence substrate for Caspase 3 and an apoptosis sensor. Overlaid on a phase contrast image..



  • Image an entire well
  • Keep organisms in focus for the duration of experiment
  • Perform sophisticated analysis of images and create time-lapse videos


  • Image neurons or stem cells grown on 3D matrices that can make focusing on the cells of interest a challenge  
  • Capture difficult-to-image thick tissue sections (at least 20 µm thick) that produce high-background fluorescence when stained
  • Analyze 3D samples by collecting a stack of crisp images in the Z-plane in as small as 100 nm increments

neuronal imaging assays analysed with high content analysis

Neuronal processes can be measured and quantified with a Neurite Outgrowth Application Module in MetaXpress Software.


laser scanning confocal system improves imaging of thick tissue sections

Mouse Intestine section stained for nuclei (green), filamentous actin prevalent in the brush border (red), goblet cells (blue)

Further Information

Visit the MetaXpress Software Application Modules to learn more about analysis for neurite outgrowth and angiogenesis application modules.  

  • Multiplex different cells in the same plate well
  • Run assays with adherent cells, suspension cells, or beads
  • Detect fluorescent labels at any wavelength







Molecular Devices brings the image acquisition and analysis for ImageXpress systems under one common platform to streamline workflow, offering a truly end-to-end solution for high-content screening.

MetaXpress® High-Content Image Acquisition & Analysis Software

  • MetaXpress Software Application Modules: turnkey solutions for your 100 most common analysis routines
  • MetaXpress Software Custom Modules: flexible toolbox for advanced assays, including 3D structure analysis with volume, intensity, and distance measurements.
  • MetaXpress Software Journals: powerful macros to analyze unique applications

AcuityXpress High-Content Informatics Software further analyzes, visualizes, and mines data.

MetaXpress® PowerCore High-Content Distributed Image Analysis Software dramatically accelerates analysis speed in a high-throughput environment.


MDCStore Data Management Solution provides users with a validated solution to securely access, manage, and depoly a high-content analysis environment.



The ImageXpress Micro  4 High-Content Imaging System offers researchers an agile imaging platform with field upgradeable confocal imaging.  For more details on the confocal system, please click here.

Additionally users can configure the instrument to fit their specific research.

For more information on product configurations and pricing, please click here.

  • Solid state white-light engine with long lifetime, suitable for most fluorescent applications
    • Lifetime: 10,000+ hrs. Wavelength: 380-680 nm
    • Included in ImageXpress Micro Imaging systems
  • Broad spectrum long-life light source is suitable for most fluorescent applications, 10 position filter wheel included for ratio imaging (Fura-2; BCECF). Compatible with multi-pass excitation cubes for additional flexibility and speed.
    • Lifetime: 10,000+ hrs. Wavelength: 340-680 nm
    • Optional upgrade for ImageXpress Micro 4 system. 
    • Filter Wheel Features
      • 10 position excitation filter wheel increases the number of preconfigured filters installed with systems to support multi-pass dichroic and emission filters in core facilities with a large variety of fluorophores in use.
      • Fast filter exchange is ideal for exciting ratiometric indicators (e.g. Fura-2; BCECF) to monitor fast cellular responses and real-time kinetics indicative of ion exchange and intracellular signaling.
    • Filter Wheel Specifications
      • Controller drives one filter wheel
      • Wheel can hold up to 10 excitation filters
      • Switching times of up to 40msec between adjacent filters
      • User adjustable speed allows the speed to load matching
      • Wheel is inserted into the light source so additional space is not required
  • Near-IR optimized solid state whitelight engine with long lifetime, suitable for most fluorescent applications enhanced for near-IR application like Cy7
    • Lifetime: 10,000+ hrs. Wavelength: 380-770 nm
    • Optional upgrade for ImageXpress Micro 4 system
  • Configurable solid state light engine is a customizable excitation source with independent control of up to 7 wavelengths. It expands flexibility of wavelength selection and is compatible with multi-pass excitation cubes for additional throughput and speed.
    • Lifetime: 10,000+ hrs. Wavelength: Customizable 380 nm to NIR
    • Optional upgrade for ImageXpress Micro 4 system

The ImageXpress Micro 4 High-Content Imaging System enables contrast imaging of unstained samples to monitor cells that may be overly sensitive to the effects of fluorescent labels or need to be monitored over time without intervention. The Phase Contrast option can be used to scrutinize cell quality before and after biochemical assays, or combined with fluorescent applications and environmental control to examine multiple parameters at once.


  • Phase optics enable high contrast, label-free cellular imaging to be performed at a variety of magnifications (4x -60x)
  • Overlay fluorescent images on unstained images gives the user another fluorophore independent visualization of cell morphology


  • Nikon 100w Pillar Diascopic Illuminator with TE-C ELWD Condenser
  • The condenser is 0.3NA with 65 mm WD and PhL, Ph1, and Ph2 selectable phase rings
  • Phase Contrast option is compatible with Environmental Control, but not with Fluidics Option

 ImageXpress Micro 4-363

The Environmental Control Option for the ImageXpress Micro 4 High-Content Imaging System can be configured to mimic conditions within a laboratory incubator (temperature control, gas mixture and humidity) to keep cells alive for long periods of time, making high-content screening kinetic and time lapse experiments a reality.


  • User-defined premixed air allows researchers to select atmospheric conditions most appropriate for their cell type (e.g. 5% or 10% CO2).
  • Temperature control of the sample plate helps maintain physiological environment.
  • Humidity regulation is maintained above sample plate to minimize evaporation of media and extend measurement time.
  • Our high resolution stage and environmental control enable fast kinetic imaging of live cells or long time lapse monitoring of each well in a plate for hours or days.


  • Accepts pressurized air from premixed tanks
  • Temperature maintained in top half of instrument: 30-40°C ± 0.5°C
  • Temperature measured near the sample plate is recorded and stored with each image in software
  • Humidity maintained above plate; evaporation < 0.5 μL/well/hour (96- or 384-well formats)
  • Compatible with Fluidics or Phase Contrast Transmitted Light options


During assay development, the Fluidics option facilitates compound addition and cell washing to characterize potency or to determine incubation times for higher throughput fixed time point assays. 

ImageXpress Micro 4-345


  • Configurable pipettor can accommodate 96- or 384-well pipette tips, compound plates and sample plates giving you the flexibility to change assay formats when you wish
  • Tip exchange option minimizes cross contamination often seen with non-contact, piezo- or pin tool-based fluidics systems
  • Integrated pipettor (3-200 µL) facilitates media replenishment or compound addition to individual wells within a microplate. Media replenishment can extend the time frame of long time lapse experiments and overcome any evaporation that may occur over time
  • Custom Light Source option can be combined with liquid handling to monitor dual-excitation wavelength intracellular ion concentration measurements (e.g. calcium, pH) to provide greater insight to ion exchange and intracellular function



  • Single-channel pipetting
  • Dispenses volumes from 3 μL to 200 μL 
  • ±1 from 3 μL to 20 μL ±5% from 20 μL to 200 μL
  • Holds two compound or media plates with optional heating
  • 96- or 384-well format FLIPR® tips
  • Comes standard with Environmental Control option
  • Not compatible with Phase Contrast Transmitted Light option




  • 4.66 megapixel scientific CMOS

  • Linear encoded voice coil driven X,Y, and Z stages have < 100 nm resolution

  • Variety of Nikon 1x- 100x air and oil immersion lenses are supported.

Part Number
Ex (nm) Dichroic (nm) EM (nm)
DAPI, Alexa Fluor 350, AMCA, BFP, Hoechst, LysoSensor Blue, Marina Blue, Pacific Blue, and sgBFP
327-404 (R)
415-950 (T)
CFP, AmCyan, SYTOX Blue, BOBO-1, BO-PRO-1
426-450 (R)
467-950 (T)
GFP, Cy2™, CyQuant, DiO, EGFP, sgGFP™, wtGFP (non-UV excitation), YOYO-1, YO-PRO-1
350-488 (R)
502-950 (T)
FITC, FAM, Alexa Fluor 488, BODIPY, Calcein, Fluo-4, MitoTracker Green, Oregon Green, Rhodamine 110, rsGFP (red-shifted GFP, S65T)
350-500 (R)
513-950 (T)
YFP, Calcium Green-1, Eosin, Fluo-3, Rhodamine 123
350-512 (R)
528-950 (T)
TRITC, Alexa Fluor 546, 5-TAMRA, Alexa Fluor 532, SpectrumOrange™, BODIPY TMR, DiI, POPO-3, PO-PRO-3, Rhodamine
350-555 (R)
569-950 (T)
Cy3™, DsRed, Alexa Fluor 555, Calcium Orange, LysoTracker Yellow, MitoTracker Orange, Phycoerythrin (PE), Rhodamine Phallicidine, and TAMRA  
350-555 (R)
569-950 (T)
Texas Red®, mCherry, 5-ROX, Alexa Fluor 568 & 594, Calcium Crimson, Cy3.5™, HcRed, Propidium Iodide, and MitoTracker Red  
350-585 (R)
601-950 (T)
Cy5™, SpectrumFRed™, Alexa Fluor 647 & 660, APC (Allophycocyanin), BODIPY 650/665, DiD, SYTO Red, DRAQ5, TOTO-3, and TO-PRO-3  
350-651 (R)

Additional filters as well as custom configurations are available upon request. 
Filter specifications are current as of 9/1/2015. 

ImageXpress Micro 4 system configuration
W x D x H
ImageXpress Micro Base Unit System
21 x 34 x 19 (Inches)
53 x 86 x 48 (Centimeters)
215 (Lbs)
95 (Kg)
Transmitted Light and Live Cell (Environmental Control)
21 x 34 x 32 (Inches)
53 x 86 x 81 (Centimeters)
220 (Lbs)
100 (Kg)
Live Cell (Environmental Control) and Fluidics
21 x 34 x 42 (Inches)
53 x 86 x 107 (Centimeters)
295 (Lbs)
134 (Kg)
Transmitted Light
21 x 34 x 32 (Inches)
53 x 86 x 81 (Centimeters)
220 (Lbs)
100 (Kg)
Live Cell (Environmental Control)
21 x 34 x 19 (Inches)
53 x 86 x 48 (Centimeters)
210 (Lbs)
95 (Kg)





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Application Notes:

Though the technical specifications of the ImageXpress Micro 4 system are different than that of our legacy ImageXpress system products, the widefield mode covers the same applications as the legacy widefield only products.


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